In this paper, an extraction method adopted by liquid chromatography by using N,N-dimethylformamide is proposed as a sample pre-treatment for the characterization of minor reference brominated and unsubstituted isatin and indirubin components by micro-Raman spectroscopy. The prized ancient dyestuff, Tyrian purple, has been extensively and widely studied by multiple spectroscopic and chromato-graphic methods alike.
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